Abstract:
Abstract
Buddharaja Kalka is a herbo-mineral drug preparation. However, there are three prescriptions
available in Sri Lanka. It is used in upper respiratory diseases, particularly in common cold and
bronchial asthma etc. The evaluation of the antibacterial activity of the Buddharaja kalka with
Makarandan anupana against Staphylococcus aureus was the main objecdtive of this study.
Sadilingam, Gandaka, Hiriyal, Manosheela, Sasyaka, Ahipena are special ingredients in the
Buddharaja Kalka and it consists of 1/3 of Sweta Chandana and 1/3 of Rakta Chandana. Three
market samples of Buddharaja Kalka from different manufactures were selected. Single
minimum human dosage in daily doses of this drug (5g) was dissolved in
Makarandanvilapambul Anupana and shaked well to get the maximum soluble liquid extract.
Nutrient broth and nutrient agar were prepared and sterilized according to manual. The tests were
performed by well diffusion method. According to the results, Amoxicillin showed 2cm - 2.5cm
clear inhibition zones of the bacterial lawn on every test drug sample. Buddharaja Kalka sample
A showed 15 mm zone of inhibition against Staphylococcus aureus while sample B showed 17
mm zone and sample C showed 18 mm zone of inhibition. These results were statistically
analyzed by using SPSS software. All the means were within accepted levels and p value was set
at P < 0.5 in comparison to Amoxicillin. All 3 samples which were tested had an antibacterial
effect in comparison to the control. It can be concluded that Buddharaja Kalkaya with
Makarandan Anupana had an anti-bacterial effect against the Staphylococcus aureus.
