Abstract:
Antioxidants are of dietary importance in preventing oxidative stress due to the production of excess
free radicals in our body leading to many diseases including cancer, atherosclerosis and diabetes as
well in aging. Despite the advances in modern medicine, plant -based remedies play an important role
in healthcare due to less side effects. Due to the side effects of NSAIDS and antibiotics, plant -based
remedies would be beneficial. This study was conducted to assess the potential use of P. campechiana
(Ratalawulu) as an antioxidant, anti-inflammatory and antibacterial agent. The aqueous leaf extract
of Ratalawulu was prepared according to the method of decoction preparation in ayurvedic medicine.
All assays were carried out according to previously published procedures. Aqueous leaf extract
of P. Baehni showed a Total Phenolic Content of 230.71±7.62 mg (PGE)/g in the Folin-Ciocalteu
method. Total flavonoid content was 140.98 ± 98.46 mg (QE)/g according to AlCl3 colorimetric assay
[PGE- Pyrogallol equivalence, QE- Quercetin equivalence]. 2,2-Diphenyl-1-picrylhydrazyl radical
scavenging activity of aqueous leaf extract of P. Baehni ranged between 1.4%-17.8% for concentration
between 0.01-0.1 mg/ml whereas ascorbic acid showed 2.46%-43.93% for the same concentration
range. Ferric ion reducing assay for aqueous leaf extract of P. Baehni showed gradual increase in
reducing property relating to antioxidant action by donating a hydrogen atom to break free radical
chains. Hydroxyl scavenging activity showed 5.73%-44.03% for concentration series of 20-100 mg/
ml whereas, Ascorbic acid ranged from 9.40%-47.06%. Hydroxyl radical is one of the potent reactive
oxygen species in the biological system. Nitric oxide scavenging anti-inflammatory assay showed
58.06%-75.36% whereas ascorbic acid showed 80.73%-91.76% for concentration series of 0.4-6.4 mg/
ml. In the human red blood cell assay, percentage protection by aqueous leaf extract of P. Baehni was
15.0%-62.4% and that of ascorbic acid was 12.1%-72.8% for 6.0-300 μg/ml. Aqueous leaf extract of P.
Baehni also showed antibacterial activity against Staphylococcus aureus. Taken together, these results
suggest the potential use of aqueous leaf extract of P. Baehni as an antioxidant, anti-inflammatory and
antibacterial agent, and merit further related investigation.
