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Diversity of midgut bacteria in larvae and females of Aedes aegypti and Aedes albopictus from Gampaha District, Sri Lanka

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dc.contributor.author Ranasinghe, K.
dc.contributor.author Gunathilaka, N.
dc.contributor.author Amarasinghe, D.
dc.contributor.author Rodrigo, W.
dc.contributor.author Udayanga, L.
dc.date.accessioned 2021-09-01T09:31:38Z
dc.date.available 2021-09-01T09:31:38Z
dc.date.issued 2021
dc.identifier.citation Parasites & Vectors.2021;14(1):433. en_US
dc.identifier.issn 1756-3305 (Undetermined)
dc.identifier.issn 1756-3305 (Linking)
dc.identifier.uri http://repository.kln.ac.lk/handle/123456789/23232
dc.description Indexed in MEDLINE en_US
dc.description.abstract BACKGROUND: The midgut microbiota of mosquitoes maintain basal immune activity and immune priming. In recent years, scientists have focused on the use of microbial communities for vector control interventions. In the present study, the midgut bacteria of larvae and adults of Aedes aegypti and Ae. albopictus were assessed using both field-collected and laboratory-reared mosquitoes from Sri Lanka. METHODS: Adults and larvae of Ae. aegypti and Ae. albopictus were collected from three selected areas in Gampaha Medical Officer of Health area, Gampaha District, Western Province, Sri Lanka. Bacterial colonies isolated from mosquito midgut dissections were identified by PCR amplification and sequencing of partial 16S rRNA gene fragments. RESULTS: Adults and larvae of Ae. aegypti and Ae. albopictus harbored 25 bacterial species. Bacillus endophyticus and Pantoea dispersa were found more frequently in field-collected Ae. aegypti and Ae. albopictus adults, respectively. The midgut bacteria of Ae. aegypti and Ae. albopictus adults (X2 = 556.167, df = 72, P < 0.001) and larvae (X2 = 633.11, df = 66, P < 0.001) were significantly different. There was a significant difference among the bacterial communities between field-collected adults (X2 = 48.974, df = 10, P < 0.001) and larvae (X2 = 84.981, df = 10, P < 0.001). Lysinibacillus sphaericus was a common species in adults and larvae of laboratory-reared Ae. aegypti. Only P. dispersa occurred in the field-collected adults of Ae. aegypti and Ae. albopictus. Species belonging to genera Terribacillus, Lysinibacillus, Agromyces and Kocuria were recorded from Aedes mosquitoes, in accordance with previously reported results. CONCLUSIONS: This study generated a comprehensive database on the culturable bacterial community found in the midgut of field-collected (Ae. aegypti and Ae. albopictus) and laboratory-reared (Ae. aegypti) mosquito larvae and adults from Sri Lanka. Data confirm that the midgut bacterial diversity in the studied mosquitoes varies according to species, developmental stage and strain (field vs laboratory). KEYWORDS: 16S rRNA gene; Aedes; Bacteria; Diversity; Midgut; Mosquitoes. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.subject Aedes aegypti en_US
dc.subject Aedes albopictus en_US
dc.title Diversity of midgut bacteria in larvae and females of Aedes aegypti and Aedes albopictus from Gampaha District, Sri Lanka en_US
dc.type Article en_US


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